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Lizarte Neto,F.S.; Tirapelli,D.P.C.; Ambrosio,S.R.; Tirapelli,C.R.; Oliveira,F.M.; Novais,P.C.; Peria,F.M.; Oliveira,H.F.; Carlotti Junior,C.G.; Tirapelli,L.F.. |
Gliomas are the most common and malignant primary brain tumors in humans. Studies have shown that classes of kaurene diterpene have anti-tumor activity related to their ability to induce apoptosis. We investigated the response of the human glioblastoma cell line U87 to treatment with ent-kaur-16-en-19-oic acid (kaurenoic acid, KA). We analyzed cell survival and the induction of apoptosis using flow cytometry and annexin V staining. Additionally, the expression of anti-apoptotic (c-FLIP and miR-21) and apoptotic (Fas, caspase-3 and caspase-8) genes was analyzed by relative quantification (real-time PCR) of mRNA levels in U87 cells that were either untreated or treated with KA (30, 50, or 70 µM) for 24, 48, and 72 h. U87 cells treated with KA demonstrated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kaurenoic acid; Glioblastoma; MiR-21; C-FLIP; Apoptosis. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100071 |
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Zhou,Yuan; Xue,Ruifeng; Wang,Jinglin; Ren,Haozhen. |
Hepatocellular carcinoma (HCC) is one of the most common primary malignant tumors of the liver worldwide. Liver resection and transplantation are currently the only effective treatments; however, recurrence and metastasis rates are still high. Previous studies have shown that the epithelial-mesenchymal transition (EMT) is a key step in HCC invasion and metastasis. Inhibition of EMT has become a new therapeutic strategy for tumors. Recently, puerarin, a well-characterized component of traditional Chinese medicine, has been isolated from Pueraria radix and exerts positive effects on many diseases, particularly cancers. In this study, CCK-8, EdU immunofluorescence, colony formation, wound healing, and migration assays were used to detect the effects of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatocellular carcinoma; Metastasis; Epithelial-mesenchymal transition; PTEN; MiR-21. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400611 |
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